Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to T3 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T3 and incubated. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured